LionHeart Epifluorescent Microscope (Agilent)

Contact Dr. Lionel Faure for training
lfaure@twu.edu

Location: SRC 105D

Description: LionheartTM FX Microscope is a compact microscopy system offering a broad range of imaging workflows using fluorescence, brightfield, color brightfield, and phase contrast channels. An environment control cover provides incubation up to 40°C and containment for CO2/O2 control, and the humidity chamber optimizes conditions for long-term live-cell imaging applications. This instrument is also connected to an automatic injector for different applications (e.g., calcium assays)

• Four different cubes for fluorescence (channel)

  • • • EX 469/55, EM 525 nm (e.g., GFP )

      • EX 377/50, EM 447/60 nm (e.g., DAPI)

      • EX 586/15, EM 647/57 nm (e.g., Texas red)

      • EX 523/40 EM 593/40 nm (e.g.,RFP)

• Phase contrast channel or brightfield

• Objectives:

  • • • 4X phase contrast 

      • 10X phase contrast

      • 20X phase contrast

      • 60X (air)

• Multivessel adapter

• Four slide holder assembly

• With this instrument, you can do the following: 

  • • • Laser autofocus option

      • Live imaging capabilities

      • Image up to 4 color channels

      • Large imaging

      • Automatic Injection

      • Z stack

      • Fast & long term kinetic (7 days)

      • Image deconvolution

      • Digital phase contract to improve contrast in BF images

      • Cellular analysis (e.g., Image statistic, cell count and confluence, mask, subpopulation analysis)

• Operate on the Gen5 plus software