CFX (Biorad)

Contact Dr. Mary Berger for training
mberger4@twu.edu

Location: SRC 320G

Description: The CFX Connect Real-Time PCR offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system provides maximal reliability & efficiency for all your RT-PCR needs.

• Max ramp temp 5℃/sec

• Average ramp rate 3.3℃/sec

• Temp range 0-100℃

• Accuracy 0.2℃

• Uniformity 0.4℃ well to well

• Gradient: 30-100℃, programmable span 1-24℃

• Range of excitation/emission wavelengths, 450/80 nm

• 3 filtered LEDs (excitation)

• 3 filtered photodiodes (detection)

• Detects 1 copy of target sequence in human genomic DNA

• sample size 1-50 µl (10-5 recommended)

• Multiplex up to 2 targets per well

Other Useful Information:

• Manual