Epifluorescent Microscope, Eclipse 90i (Nikon)

Contact Dr. Lionel Faure for training
lfaure@twu.edu

Location: SRC 320K

Description: The 90i is ideal for fluorescent and/or bright-field images of whole tissues and small organisms. Three single filters allow fluorescence detection in the widely used DAPI, FITC, and TRITC ranges. Great microscope for histology, cytochemistry, and immunocytochemistry (stain or fluorescent).

• Objectives: 4X, 10X, 20X, 40X 60X and 100X (Oil)

• Filters:

  • • • • DAPI (375 to 450 nm)

        • GFP (470 to 570 nm)

        • TRITC/ Cy 5/Texas Red  (580 to 700 nm)

• Excitation  by epifluorescence is LEDs with a range of about 375 to 700 nm

• Operates on NIS element AR software

• All systems are motorized except for the slide movement in the field.

• Fast CMOS Camera that can be used for density quantification and is high resolution. The system can do color images.